Influence of Auxiliary Enzymes on the Spectrophotometric Measurement of Alanine Aminotransferase and Aspartate Aminotransferase Activities
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چکیده
منابع مشابه
Influence of auxiliary enzymes on the spectrophotometric measurement of alanine aminotransferase and aspartate aminotransferase activities.
We investigated the enzyme activity of the blank in the spectrophotometric determination of the aminotransferase activities and aspartate aminotransferase activity. 6 lactate dehydrogenase and 3 malate dehydrogenase preparations from different manufactures and from different organs showed additional and contaminating activity. The additional activity depends upon the 2-oxoglutarate concentratio...
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The catalytic activity concentrations of aspartate aminotransferase (EC 2.6.1.1) and alanine aminotransferase (EC 2.6.1.2) in several commercial control sera were determined at three different temperatures. Although these sera were of various biological origins, the temperature-conversion factors calculated are nearly identical to those for native human sera. The proportional increase of the ac...
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Conditions for accurate measurement of catalytic activity of aspartate aminotransferase and alanine aminotransferase in human serum have been reinvestigated. The basic variables (kind of buffer, buffer concentration, pH, ion effects, and the influence of pyridoxal-5-phosphate) can now be considered optimized. On this basis, the kinetic parameters of both aminotransferases were determined, i.e.,...
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The determination of frequency value (percentile limits) and the classification of the different variation factors allow us to define more and more homogeneous subpopulations as we use these factors for sorting. Using as our study population those persons coming to the Centre for Preventive Medicine, we were able to: (a) Describe and measure the significance and importance of physiological vari...
متن کاملInfluence of pyridoxal-5'-phosphate on the determination of the alanine aminotransferase and aspartate aminotransferase of commercial test sera.
The influence of pyridoxal-5'-phosphate on the activities of alanine aminotransferase and aspartate aminotransferase from commercial test sera was investigated. Enhancement of the activity of alanine aminotransferase by the coenzyme is about 20% when 0.1 mmol/1 of pyridoxal-S'-phosphate is added to the reaction medium. This concentration seems sufficient for full saturation of the apoenzyme. Fo...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1976
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1976.14.1-12.53